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Mineralisation of biological samples in ultratrace analysis
Kryštofová, Aneta ; Komendová, Renata (referee) ; Vašinová Galiová, Michaela (advisor)
Cancer is one of the most serious and most common diseases in today's population. For this reason, research is being carried out to discover new ways of treating cancer. One of the frequently used treatments is chemotherapy. However, due to the side effects on the health of cancer patients of the currently known chemotherapeutic agents, new substances with anticancer effect are still being developed. In this bachelor thesis, the mineralization processes of cancer cells injected with chemotherapeutic agents were compared. One of the digestion approaches was wet digestion using nitric acid and hydrogen peroxide in different ratios. This method of digestion was compared with digestion using a microwave device, where mineralization was most efficient. Indeed, mineralization of tumor cells was the first step in determining the efficacy of a chemotherapeutic drug in a particular cell line. The ICP-MS method was used to analyse the tumour cells, prior to which the cell samples had to be transferred into solution and this was done by digestion. Unfortunately, due to the variability of the biological samples used, it was not possible to clearly assess whether the hydrogen peroxide contained in the digestion mixture contributed to the efficiency of mineralisation.
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Determination of selected elements in coffee samples using ICP-MS method
Švecová, Pavla ; Hraníček, Jakub (advisor) ; Červený, Václav (referee)
EN The main aim of this thesis is focused on the determination of selected elements (manganese, copper, zinc, iron) in trace levels in selected samples of grained and instant coffee. Four samples of grained coffee and one sample of instant coffee were taken for analysis. Firstly, all samples were digested in a microwave digestion system and then analysed by inductively coupled plasma mass spectrometry to determine the concentration of selected elements. Except this, one sample of grained coffee was taken for two additional experiments. The aim of these experiments was to investigate the influence of the extraction time and temperature of water used for extractions, on the element concentrations in liquid phases. The content of elements in the analysed samples shows relatively wide range of values (7.58 - 34.4, 0.21 - 13.6, 1.58 - 5.35 and 12.2 - 55.8 µg/g for manganese, copper, zinc and iron, respectively). Generally, the lowest concentrations of all elements were detected in sample of instant coffee, which cannot be regarded as a valuable source of trace elements for humans. The first additional experiment shows that the concentrations of elements in grained coffee is much higher than in its extractions. With increasing time of extraction, the concentration of elements increased as well (except of...
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